Distortion was also observed in samples that contains lipids, especially in the Hoki roe homogenate. Pokhariyal et al. [forty nine] noted which the complexity of protein bands from the SDS-Webpage was elevated from the existence of lipid, which resulted in distortion of protein bands.
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The 2 protein bands from 40 kDa to 30 kDa were being absent soon after 24 h incubation at forty five °C. For the rest of the bands, several concentrations of all three proteases retained protein bands equivalent to their initial molar masses (Figure 6a,b). The existence of bands at minimal molecular weight in fish roe prior to hydrolysis was unanticipated. While in the report by Ghelichi et al. [eight], slight peaks of a comparatively very low molecular pounds vary (three–14 kDa) have been observed in defatted carp roe homogenate, probably resulting from an autolytic system taking place in fish roe and/or even the defatting approach leading to the formation of low-molecular-body weight peptides.
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